MYL3/Myosin light chain 3 Antibody, AbBy Fluor-405 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-2240r-bf405 |
| Product Name | MYL3/Myosin light chain 3 Antibody, AbBy Fluor-405 Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Human, Mouse, Rat, Bovine, Dog, Pig, Chicken |
| Specifications | |
| Conjugation | AbBy Fluor-405 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human MLC/Myosin light chain 3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4634 |
| Synonyms | Cardiac myosin light chain 1; Cardiac myosin light chain 1; CMH8; cmlc1; MLC; MLC1SB; MLC1V; MYL 3; MYL3; Myosin light chain 1 slow twitch muscle B/ventricular isoform; Myosin light chain 1 slow-twitch muscle B/ventricular isoform; Myosin light polypeptide 3; Slow skeletal ventricular myosin alkali light chain 3; Ventricular/slow twitch myosin alkali light chain; VLC1; MYL3_HUMAN. |
| Background | Myosin is the major component of thick muscle filaments, and is a long asymmetric molecule containing a globular head and a long tail. The molecule consists of two heavy chains each ~200,000 daltons, and four light chains each ~16,000 - 21,000 daltons. Activation of smooth and cardiac muscle primarily involves pathways which increase calcium and myosin phosphorylation resulting in contraction. Myosin light chain phosphatase acts to regulate muscle contraction by dephosphorylating activated myosin light chain. Human myosin light chain has clinical application as a cardiac marker. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is MYL3/Myosin light chain 3 Antibody, AbBy Fluor-405 Conjugated (bs-2240R-BF405) validated for, and at what dilution?
bs-2240R-BF405 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does MYL3/Myosin light chain 3 Antibody, AbBy Fluor-405 Conjugated (bs-2240R-BF405) react with?
Tested reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Chicken.
How should bs-2240R-BF405 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise MYL3/Myosin light chain 3 Antibody, AbBy Fluor-405 Conjugated (bs-2240R-BF405)?
KLH conjugated synthetic peptide derived from human MLC/Myosin light chain 3
What are the excitation and emission wavelengths of the AbBy Fluor-405 conjugate (bs-2240R-BF405)?
AbBy Fluor-405 on bs-2240R-BF405: excitation / emission 401nm/421nm.