ACA11 Polyclonal Antibody, AbBy Fluor-680 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-2249R-BF680 |
| Product Name | ACA11 Polyclonal Antibody, AbBy Fluor-680 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Arabidopsis Thaliana |
| Specifications | |
| Conjugation | AbBy Fluor-680 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from Arabidopsis thaliana ACA11 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 677770 |
| Swiss Prot | O22218 |
| Subcellular location | Cell membrane |
| Synonyms | calcium-transporting ATPase; Putative calcium-transporting ATPase 11, plasma membrane-type; Ca2+-ATPase isoform 11; autoinhibited Ca2+-ATPase 11Arabidopsis thaliana; ACA11_ARATH. |
| Background | In plant cells, the vacuole functions as a major calcium store. Although a calmodulin-regulated Ca2+-ATPase (ACA4) is known to be present in prevacuolar compartments, the presence of an ACA-type Ca2+-ATPase in the mature vacuole of a plant cell has not been verified. Here we provide evidence that ACA11 localizes to the vacuole membrane. ACA11 tagged with GFP was expressed in stable transgenic plants, and visualized in root cells and protoplasts by confocal microscopy. A Ca2+-ATPase function for ACA11 was confirmed by complementation of yeast mutants. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is ACA11 Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-2249R-BF680) validated for, and at what dilution?
bs-2249R-BF680 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-2249R-BF680 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ACA11 Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-2249R-BF680)?
KLH conjugated synthetic peptide derived from Arabidopsis thaliana ACA11
What are the excitation and emission wavelengths of the AbBy Fluor-680 conjugate (bs-2249R-BF680)?
AbBy Fluor-680 on bs-2249R-BF680: excitation / emission 679nm/702nm.