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CPSF4 Polyclonal Antibody, Cy5 Conjugated

Applications

  • WB

Reactivity

  • Human
  • Mouse

Predicted Reactivity

  • Rat
  • Dog
  • Cow
  • Pig
  • Horse
  • Chicken
  • Rabbit
Overview
Catalog # bs-2253R-Cy5
Product Name CPSF4 Polyclonal Antibody, Cy5 Conjugated
Applications
WB
Reactivity Human, Mouse
Predicted Reactivity Rat, Dog, Cow, Pig, Horse, Chicken, Rabbit
Specifications
Conjugation Cy5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human CPSF4
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 10898
Swiss Prot O95639
Subcellular location Nucleus
Synonyms NAR; NEB1; CPSF30; Cleavage and polyadenylation specificity factor subunit 4; Cleavage and polyadenylation specificity factor 30 kDa subunit; CPSF 30 kDa subunit; NS1 effector domain-binding protein 1; Neb-1; No arches homolog; CPSF4
Background Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. CPSF4 binds RNA polymers with a preference for poly(U).
Application Dilution
WB 1:300-5000

Frequently asked questions

Which applications is CPSF4 Polyclonal Antibody, Cy5 Conjugated (bs-2253R-Cy5) validated for, and at what dilution?

bs-2253R-Cy5 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does CPSF4 Polyclonal Antibody, Cy5 Conjugated (bs-2253R-Cy5) react with?

Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Cow, Pig, Horse, Chicken, Rabbit.

How should bs-2253R-Cy5 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise CPSF4 Polyclonal Antibody, Cy5 Conjugated (bs-2253R-Cy5)?

KLH conjugated synthetic peptide derived from human CPSF4

What are the excitation and emission wavelengths of the Cy5 conjugate (bs-2253R-Cy5)?

Cy5 on bs-2253R-Cy5: excitation / emission 625,650nm/670nm.