DGAT2 Polyclonal Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-22551r-100uL |
| Product Name | DGAT2 Polyclonal Antibody |
| Applications |
WB
IHC-P
IHC-F
IF
|
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse, Dog, Cow, Sheep, Horse |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human DGAT2 |
| Immunogen Range | 201-300/388 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 84649 |
| Swiss Prot | Q96PD7 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | DGAT2_HUMAN; Diacylglycerol O acyltransferase like protein 2; Diacylglycerol O-acyltransferase 2; Diacylglycerol O-acyltransferase homolog 2 (mouse); Diacylglycerol O-acyltransferase homolog 2; Diacylglycerol O-acyltransferase-like protein 2 isoform 1; Diglyceride acyltransferase 2; DKFZp686A15125; GS1999full; HMFN1045. |
| Background | Glucose and insulin are anabolic signals which upregulate the transcriptions of a series of lipogenic enzymes to convert excess carbohydrate into triglycerides for efficient energy storage. Acyl-coenzyme A:diacylglycerol acyltransferase, also known as DGAT1 and ARGP1, is a microsomal enzyme that assists in the synthesis of fatty acids into triglycerides. DGAT1 catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol (DAG) and fatty acyl CoA as substrates. DGAT1 plays a fundamental role in the metabolism of cellular diacylglycerol and is important in higher eukaryotes for physiologic processes involving triacylglycerol metabolism, such as intestinal fat absorption, lipoprotein assembly, adipose tissue form-ation and lactation. DGAT2, which has no homology to DGAT1, differs from DGAT1 in that its activity has been shown to be inhibited by MgCl in an in vitro assay. DGAT2 is expressed primarily in liver and white adipose tissue, which suggests that it plays an important role in mammalian triglyceride metabolism. |
| Application Dilution | |
| WB | =1:500-2000 |
| IHC-P | IHC-P=1:100-500 |
| IHC-F | IHC-F=1:100-500 |
| IF | IF=1:100-500 |
Frequently asked questions
Which applications is DGAT2 Polyclonal Antibody (bs-22551R) validated for, and at what dilution?
bs-22551R is validated for WB (WB=1:500-2000); IHC-P (IHC-P=1:100-500); IHC-F (IHC-F=1:100-500); IF (IF=1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does DGAT2 Polyclonal Antibody (bs-22551R) react with?
Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Dog, Cow, Sheep, Horse.
How should bs-22551R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise DGAT2 Polyclonal Antibody (bs-22551R)?
KLH conjugated synthetic peptide derived from human DGAT2 Immunogen range: 201-300/388.