UBF1 Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with UBF1 Polyclonal Antibody, Unconjugated (bs-22564R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with UBF1 Polyclonal Antibody, Unconjugated (bs-22564R) at 1:200 overnight at 4\u00b0C, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with UBF1 Polyclonal Antibody, Unconjugated (bs-22564R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with UBF1 Polyclonal Antibody, Unconjugated (bs-22564R) at 1:200 overnight at 4\u00b0C, DAB staining.

Applications

IHC-P

Reactivity

Mouse
Rat

Predicted Reactivity

Human
Dog
Cow
Sheep
Pig
Horse

Overview
Catalog #	bs-22564R
Product Name	UBF1 Polyclonal Antibody
Applications	IHC-P
Reactivity	Mouse, Rat
Predicted Reactivity	Human, Dog, Cow, Sheep, Pig, Horse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human UBF1
Immunogen Range	611-710/764
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	7343
Swiss Prot	P17480
Subcellular location	Nucleus
Synonyms	UBF; NOR 90; Nucleolar transcription factor 1; UBF 1; UBF-1; UBF; UBTF; Autoantigen NOR-90; Upstream-binding factor 1; Upstream binding factor 1; upstream binding transcription factor, RNA polymerase I; UBF1_HUMAN.
Background	Recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I through cooperative interactions with the transcription factor SL1/TIF-IB complex. It binds specifically to the upstream control element.
Application Dilution
IHC-P	1:200-400

Applications

Reactivity

Predicted Reactivity

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