Contact us at 1.800.501.7654 or info@biossusa.com

MenuSearch
Help

Calreticulin Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Calreticulin) Polyclonal Antibody, Unconjugated (bs-22576R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Calreticulin) Polyclonal Antibody, Unconjugated (bs-22576R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Calreticulin) Polyclonal Antibody, Unconjugated (bs-22576R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Calreticulin) Polyclonal Antibody, Unconjugated (bs-22576R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Calreticulin) Polyclonal Antibody, Unconjugated (bs-22576R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Calreticulin) Polyclonal Antibody, Unconjugated (bs-22576R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Bioss unconjugated antibody vial

Applications

  • WB
  • IHC-P

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Cow
  • Sheep
  • Horse
  • Chicken
  • Pig
Overview
Catalog # bs-22576R
Product Name Calreticulin Polyclonal Antibody
Applications
WB IHC-P
Reactivity Human, Mouse, Rat
Predicted Reactivity Cow, Sheep, Horse, Chicken, Pig
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Calreticulin
Clonality Polyclonal
Isotype IgG
Concentration Lot Dependent
Purification purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 811
Swiss Prot P27797
Subcellular location Cytoplasm, Secreted, Cell membrane, Extracellular matrix
Synonyms Autoantigen RO; CALR; CALR protein; CALR_HUMAN; cC1qR; CRP55; CRTC; Endoplasmic reticulum resident protein 60; ERp60; grp60; HACBP; RO; Sicca syndrome antigen A; SSA.
Application Dilution
WB =1:500-2000
IHC-P IHC-P=1:100-500

Frequently asked questions

Which applications is Calreticulin Polyclonal Antibody (bs-22576R) validated for, and at what dilution?

bs-22576R is validated for WB (WB=1:500-2000); IHC-P (IHC-P=1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Calreticulin Polyclonal Antibody (bs-22576R) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Cow, Sheep, Horse, Chicken, Pig.

How should bs-22576R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Calreticulin Polyclonal Antibody (bs-22576R)?

KLH conjugated synthetic peptide derived from human Calreticulin

Search our catalog of hundreds of thousands of products