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ADAM10 Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (ADAM10) Polyclonal Antibody, Unconjugated (bs-22676R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (ADAM10) Polyclonal Antibody, Unconjugated (bs-22676R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (ADAM10) Polyclonal Antibody, Unconjugated (bs-22676R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (ADAM10) Polyclonal Antibody, Unconjugated (bs-22676R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (ADAM10) Polyclonal Antibody, Unconjugated (bs-22676R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (ADAM10) Polyclonal Antibody, Unconjugated (bs-22676R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Bioss unconjugated antibody vial

Applications

  • IHC-P
  • IHC-F
  • IF

Reactivity

  • Mouse
  • Rat

Predicted Reactivity

  • Human
  • Dog
  • Cow
  • Sheep
  • Pig
  • Chicken
  • Rabbit
Overview
Catalog # bs-22676r
Product Name ADAM10 Polyclonal Antibody
Applications
IHC-P IHC-F IF
Reactivity Mouse, Rat
Predicted Reactivity Human, Dog, Cow, Sheep, Pig, Chicken, Rabbit
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human ADAM10/
Clonality Polyclonal
Isotype IgG
Concentration 1mg/ml
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 102
Swiss Prot O14672
Subcellular location Cytoplasm, Cell membrane
Synonyms A disintegrin and metalloprotease domain 10; A Disintegrin And Metalloproteinase Domain 10; ADAM 10; ADAM metallopeptidase domain 10; CD 156c; CD156c; CD156c antigen; CDw156 antibody HsT 18717; HsT18717; kuz; kuzbanian; Kuzbanian protein homolog; MADM; Mammalian disintegrin metalloprotease; ADA10_HUMAN; RAK; kuz; AD10.
Application Dilution
IHC-P =1:100-500
IHC-F IHC-F=1:100-500
IF IF=1:100-500

Frequently asked questions

Which applications is ADAM10 Polyclonal Antibody (bs-22676R) validated for, and at what dilution?

bs-22676R is validated for IHC-P (IHC-P=1:100-500); IHC-F (IHC-F=1:100-500); IF (IF=1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does ADAM10 Polyclonal Antibody (bs-22676R) react with?

Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Cow, Sheep, Pig, Chicken, Rabbit.

How should bs-22676R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise ADAM10 Polyclonal Antibody (bs-22676R)?

KLH conjugated synthetic peptide derived from human ADAM10/

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