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BHMT

Paraformaldehyde-fixed, paraffin embedded (Human kidney carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (BHMT) Polyclonal Antibody, Unconjugated (bs-22698R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human kidney carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (BHMT) Polyclonal Antibody, Unconjugated (bs-22698R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Lane 1: Mouse Liver Lysates; Lane 2: Rat Liver Lysates. Probed with BHMT polyclonal Antibody, unconjugated (bs-22698R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
Lane 1: Mouse Liver Lysates; Lane 2: Rat Liver Lysates. Probed with BHMT polyclonal Antibody, unconjugated (bs-22698R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
Bioss unconjugated antibody vial

Applications

  • WB
  • IHC-P
  • IHC-F
  • IF

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
  • Dog
  • Cow
  • Sheep
  • Pig
Overview
Catalog # bs-22698r
Product Name BHMT
Applications
WB IHC-P IHC-F IF
Reactivity Human
Predicted Reactivity Mouse, Rat, Dog, Cow, Sheep, Pig
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human BHMT
Immunogen Range 310-406/406
Clonality Polyclonal
Isotype IgG
Concentration 1mg/ml
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 635
Swiss Prot Q93088
Subcellular location Cytoplasm
Synonyms Betaine homocysteine methyltransferase ; Betaine homocysteine S methyltransferase 1 ; Betaine homocysteine S methyltransferase ; Betaine--homocysteine S-methyltransferase 1 ; BHMT1 ; BHMT1_HUMAN ; EC 2.1.1.5 ; Epididymis secretory sperm binding protein Li 61p ; HEL S 61p.
Background Involved in the regulation of homocysteine metabolism. Converts betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline.
Application Dilution
WB =1:500-2000
IHC-P IHC-P=1:100-500
IHC-F IHC-F=1:100-500
IF IF=1:100-500

Frequently asked questions

Which applications is BHMT (bs-22698R) validated for, and at what dilution?

bs-22698R is validated for WB (WB=1:500-2000); IHC-P (IHC-P=1:100-500); IHC-F (IHC-F=1:100-500); IF (IF=1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does BHMT (bs-22698R) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Sheep, Pig.

How should bs-22698R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise BHMT (bs-22698R)?

KLH conjugated synthetic peptide derived from human BHMT Immunogen range: 310-406/406.

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