PAX7 Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Mouse embryo; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PAX7 Polyclonal Antibody, Unconjugated (bs-22741R) at 1:2000 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse embryo; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PAX7 Polyclonal Antibody, Unconjugated (bs-22741R) at 1:2000 overnight at 4\u00b0C, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Mouse uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PAX7 Polyclonal Antibody, Unconjugated (bs-22741R) at 1:2000 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PAX7 Polyclonal Antibody, Unconjugated (bs-22741R) at 1:2000 overnight at 4\u00b0C, DAB staining.

Applications

WB
IHC-P

Reactivity

Mouse
Rat

Predicted Reactivity

Human
Cow
Sheep
Pig
Horse
Chicken
Rabbit

Overview
Catalog #	bs-22741R
Product Name	PAX7 Polyclonal Antibody
Applications	WB, IHC-P
Reactivity	Mouse, Rat
Predicted Reactivity	Human, Cow, Sheep, Pig, Horse, Chicken, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human PAX7
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	5081
Swiss Prot	P23759
Subcellular location	Nucleus
Synonyms	Paired box gene 7; FLJ37460; HUP1; OTTHUMP00000002534; Paired box 7; Paired box homeotic gene 7; Paired box protein Pax-7; Paired domain gene 7; Paired domain gene HuP1; PAX7 transcriptional factor; PAX7/FKHR fusion gene, included; PAX7B; RGD1564360; PAX7_HUMAN.
Background	Probable transcription factor. May have a role in myogenesis.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400