Peroxiredoxin-5 Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Peroxiredoxin-5 Polyclonal Antibody, Unconjugated (bs-22754R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Peroxiredoxin-5 Polyclonal Antibody, Unconjugated (bs-22754R) at 1:200 overnight at 4\u00b0C, DAB staining.

Applications

IHC-P
IHC-F
IF

Reactivity

Predicted Reactivity

Human
Mouse
Dog
Cow
Pig
Horse

Overview
Catalog #	bs-22754R
Product Name	Peroxiredoxin-5 Polyclonal Antibody
Applications	IHC-P, IHC-F, IF
Reactivity	Rat
Predicted Reactivity	Human, Mouse, Dog, Cow, Pig, Horse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Peroxiredoxin-5
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	25824
Swiss Prot	P30044
Subcellular location	Cytoplasm
Synonyms	Alu corepressor 1;Antioxidant enzyme B166;AOEB166;Liver tissue 2D-page spot 71B;PLP;Peroxiredoxin V; Prx-V;Peroxisomal antioxidant enzyme;TPx type VI;Thioredoxin peroxidase PMP20;PRDX5;PRDX5_HUMAN
Background	Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events.
Application Dilution
IHC-P	1:200-400
IHC-F
IF