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IL-13Ra2 Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with IL-13Ra2 Polyclonal Antibody, Unconjugated (bs-22785R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with IL-13Ra2 Polyclonal Antibody, Unconjugated (bs-22785R) at 1:200 overnight at 4\u00b0C, DAB staining.
Lane 1: Human U251 cell lysates; Lane 2: Human HeLa cell lysates probed with IL13RA2 Polyclonal Antibody, Unconjugated (bs-22785R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human U251 cell lysates; Lane 2: Human HeLa cell lysates probed with IL13RA2 Polyclonal Antibody, Unconjugated (bs-22785R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

  • IHC-P

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
Overview
Catalog # bs-22785R
Product Name IL-13Ra2 Polyclonal Antibody
Applications IHC-P
Reactivity Human
Predicted Reactivity Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from mouse IL-13Ra2
Immunogen Range 21-120/383
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 16165
Swiss Prot O88786
Subcellular location Cell membrane
Synonyms Cancer/testis antigen 19; CD213a2; CT19; I13R2_HUMAN; IL-13 receptor subunit alpha-2; IL-13R subunit alpha-2; IL-13R-alpha-2; IL-13RA2; IL13 receptor; IL13BP; IL13R; Il13ra2; Interleukin 13 binding protein; Interleukin 13 receptor alpha 2 chain; Interleukin-13 receptor subunit alpha-2; Interleukin-13-binding protein.
Background Binds as a monomer with high affinity to interleukin-13 (IL13), but not to interleukin-4 (IL4).
Application Dilution
IHC-P 1:200-400

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