RAR alpha Polyclonal Antibody, PE-Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-22843R-PE-Cy5 |
| Product Name | RAR alpha Polyclonal Antibody, PE-Cy5 Conjugated |
| Applications |
WB
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | PE-Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human RAR alpha |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5914 |
| Swiss Prot | P11416 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | Acute Promelocytic Leukemia Breakpoint Cluster Region; Acute Promelocytic Leukemia Breakpoint Cluster Region; NR1B1; Nuclear mitotic apparatus protein retinoic acid receptor alpha fusion protein; Nuclear receptor subfamily 1 group B member 1; Nucleophosmin retinoic acid receptor alpha fusion protein NPM RAR long form; RAR A; RAR alpha; RAR alpha; RAR alpha form; RAR alpha form; RAR; RAR-alpha; RARA; RARA; RARA protein; RARA/PML Fusion Gene; RARA/PML Fusion Gene; RARA_HUMAN; RARalpha; RARalpha1; Retinoic acid receptor alpha; Retinoic acid receptor alpha polypeptide |
| Background | Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone acetylation, chromatin condensation and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. RARA plays an essential role in the regulation of retinoic acid-induced germ cell development during spermatogenesis. Has a role in the survival of early spermatocytes at the beginning prophase of meiosis. In Sertoli cells, may promote the survival and development of early meiotic prophase spermatocytes. In concert with RARG, required for skeletal growth, matrix homeostasis and growth plate function (By similarity). Regulates expression of target genes in a ligand-dependent manner by recruiting chromatin complexes containing MLL5. Mediates retinoic acid-induced granulopoiesis. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is RAR alpha Polyclonal Antibody, PE-Cy5 Conjugated (bs-22843R-PE-Cy5) validated for, and at what dilution?
bs-22843R-PE-Cy5 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does RAR alpha Polyclonal Antibody, PE-Cy5 Conjugated (bs-22843R-PE-Cy5) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit.
How should bs-22843R-PE-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise RAR alpha Polyclonal Antibody, PE-Cy5 Conjugated (bs-22843R-PE-Cy5)?
KLH conjugated synthetic peptide derived from human RAR alpha
What are the excitation and emission wavelengths of the PE-Cy5 conjugate (bs-22843R-PE-Cy5)?
PE-Cy5 on bs-22843R-PE-Cy5: excitation / emission 488nm/670nm.