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Cytochrome C Polyclonal Antibody, PerCP Conjugated

Applications

  • WB

Reactivity

  • Mouse
  • Rat

Predicted Reactivity

  • Cow
  • Pig
  • Horse
Overview
Catalog # bs-22900R-PerCP-100uL
Product Name Cytochrome C Polyclonal Antibody, PerCP Conjugated
Applications
WB
Reactivity Mouse, Rat
Predicted Reactivity Cow, Pig, Horse
Specifications
Conjugation PerCP
Host Rabbit
Source Cytochrome c from bovine heart
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 54205
Swiss Prot P99999
Subcellular location Cytoplasm, Cell membrane
Synonyms CytC; CYC; CYCS; Cytochrome c somatic; HCS; CYC_HUMAN; CYC_MOUSE; Cytochrome-c; MSA06; THC4.
Background Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.
Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.
Application Dilution
WB

Frequently asked questions

Which applications is Cytochrome C Polyclonal Antibody, PerCP Conjugated (bs-22900R-PerCP) validated for, and at what dilution?

bs-22900R-PerCP is validated for WB.

Which species does Cytochrome C Polyclonal Antibody, PerCP Conjugated (bs-22900R-PerCP) react with?

Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Cow, Pig, Horse.

How should bs-22900R-PerCP be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Cytochrome C Polyclonal Antibody, PerCP Conjugated (bs-22900R-PerCP)?

Cytochrome c from bovine heart

What are the excitation and emission wavelengths of the PerCP conjugate (bs-22900R-PerCP)?

PerCP on bs-22900R-PerCP: excitation / emission 482nm/677nm.