iNOS Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Rat colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with iNOS Polyclonal Antibody, Unconjugated (bs-22924R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with iNOS Polyclonal Antibody, Unconjugated (bs-22924R) at 1:200 overnight at 4\u00b0C, DAB staining.

Applications

IHC-P
IHC-F
IF

Reactivity

Predicted Reactivity

Mouse
Rabbit

Overview
Catalog #	bs-22924R
Product Name	iNOS Polyclonal Antibody
Applications	IHC-P, IHC-F, IF
Reactivity	Rat
Predicted Reactivity	Mouse, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from mouse iNOS
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	18126
Swiss Prot	P29477
Subcellular location	Cytoplasm, Nucleus
Synonyms	i NOS; I-NOS; Nitric Oxide Synthase, Inducible; HEP NOS; Hepatocyte NOS; HEPNOS; Inducible nitric oxide synthase; Inducible NO synthase; Inducible NOS; INOS; Inosl; MAC NOS; Macrophage NOS; Nitric oxide synthase 2 inducible macrophage; Nitric oxide synthase 2A (inducible hepatocytes); Nitric oxide synthase inducible; NOS 2; NOS 2A; NOS; Nos II; NOS type II; Nos2; NOS2A; NOS2_HUMAN.
Background	Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2.
Application Dilution
IHC-P	1:200-400
IHC-F
IF