Bak Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-22928R-Cy3-100uL |
| Product Name | Bak Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Bak |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 578 |
| Swiss Prot | O08734 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | Apoptosis Regulator Bak; BAK 1; BAK; BAK like; Bak NT; BAK1; Bcl 2 homologous antagonist/killer; Bcl 2 like 7 protein; Bcl2 homologous antagonist killer; Bcl2 like 7 Protein; BCL2-antagonist/killer 1; BCL2L7; CDN 1; CDN1; Cell death inhibitor 1; MGC117255; MGC3887; NBak; Pro apoptotic protein BAK; BAK_HUMAN; BAK_MOUSE |
| Background | The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form oligomers or heterodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. This protein also interacts with the tumor suppressor P53 after exposure to cell stress. [provided by RefSeq, Jul 2008] |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is Bak Polyclonal Antibody, Cy3 Conjugated (bs-22928R-Cy3) validated for, and at what dilution?
bs-22928R-Cy3 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Bak Polyclonal Antibody, Cy3 Conjugated (bs-22928R-Cy3) react with?
Tested reactivity: Human.
How should bs-22928R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Bak Polyclonal Antibody, Cy3 Conjugated (bs-22928R-Cy3)?
KLH conjugated synthetic peptide derived from human Bak
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-22928R-Cy3)?
Cy3 on bs-22928R-Cy3: excitation / emission 512,550nm/570,615nm.