RSL1D1 Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Rat uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RSL1D1 Polyclonal Antibody, Unconjugated (bs-22952R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RSL1D1 Polyclonal Antibody, Unconjugated (bs-22952R) at 1:200 overnight at 4\u00b0C, DAB staining.

Lane 1: Rat Heart lysates; Lane 2: Mouse Heart lysates; Lane 3: Rat Muscle lysates; Lane 4: Mouse Muscle lysates; Lane 5: Rate Placenta lysates; Lane 6: Mouse Placenta lysates; Lane 7: Rat Cerebrum lysates; Lane 8: Mouse Cerebrum lysates; Lane 9: Rat Testis lysates; Lane 10: Mouse Testis lysates; Lane 11: Rat Uterus lysates; Lane 12: Mouse Uterus lysates probed with RSL1D1 Polyclonal Antibody, Unconjugated (bs-22952R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
IHC-P
IHC-F
IF

Reactivity

Mouse
Rat

Overview
Catalog #	bs-22952R
Product Name	RSL1D1 Polyclonal Antibody
Applications	WB, IHC-P, IHC-F, IF
Reactivity	Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from mouse RSL1D1
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	66409
Swiss Prot	Q8BVY0
Subcellular location	Nucleus
Synonyms	CATX 11; CATX-11; CATX11; Cellular senescence inhibited gene protein; L12; PBK1; CSIG; L12; PBK1; Protein PBK1; Ribosomal L1 domain-containing protein 1; RL1D1_HUMAN; Ribosomal L1 domain containing 1.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IHC-F
IF