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CD162 Polyclonal Antibody, RBITC Conjugated

Applications

  • WB
  • FCM

Reactivity

  • Human
Overview
Catalog # bs-2301R-RBITC
Product Name CD162 Polyclonal Antibody, RBITC Conjugated
Applications
WB FCM
Reactivity Human
Specifications
Conjugation RBITC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human PSGL-1/CD162
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 6404
Swiss Prot Q14242
Subcellular location Cell membrane
Synonyms CLA; CD162; PSGL1; PSGL-1; P-selectin glycoprotein ligand 1; Selectin P ligand; SELPLG
Background A SLe(x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. Critical for the initial leukocyte capture.
Application Dilution
WB 1:300-5000
FCM 1:20-100

Frequently asked questions

Which applications is CD162 Polyclonal Antibody, RBITC Conjugated (bs-2301R-RBITC) validated for, and at what dilution?

bs-2301R-RBITC is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does CD162 Polyclonal Antibody, RBITC Conjugated (bs-2301R-RBITC) react with?

Tested reactivity: Human.

How should bs-2301R-RBITC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise CD162 Polyclonal Antibody, RBITC Conjugated (bs-2301R-RBITC)?

KLH conjugated synthetic peptide derived from human PSGL-1/CD162

What are the excitation and emission wavelengths of the RBITC conjugate (bs-2301R-RBITC)?

RBITC on bs-2301R-RBITC: excitation / emission 540nm/625nm.