Dnmt3a Polyclonal Antibody, AbBy Fluor-350 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-23029R-BF350-100uL |
| Product Name | Dnmt3a Polyclonal Antibody, AbBy Fluor-350 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Cow, Sheep, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-350 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human DNMT-3 alpha |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1788 |
| Swiss Prot | Q9Y6K1 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | DNA (cytosine 5 ) methyltransferase 3 alpha; DNA (cytosine 5) methyltransferase 3A; DNA cytosine methyltransferase 3A2; DNA methyltransferase 3a; DNA methyltransferase HsaIIIA; DNA MTase HsaI; DNA MTase HsaIIIA; DNMT 3a; DNMT; Dnmt3a; DNMT3A2; M HsaIIIA; M.HsaIIIA; MCMT; Methyl CpG binding domain protein 3a. |
| Background | The tumor organization has the DNA methylation disorder, including and damps cancer gene high methylation DNA with the cell multiplication cycle close related cancer gene low methylation the methyl transferase (Dnmt) participation methylation the formation (is mainly Dnmt3a and Dnmt3b) and the maintenance (is mainly Dnmt1) (isoform CRA_b) (DNA methyltransferase MmuIIIA) (DNA MTase MmuIIIA) |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Dnmt3a Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-23029R-BF350) validated for, and at what dilution?
bs-23029R-BF350 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Dnmt3a Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-23029R-BF350) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Sheep, Rabbit.
How should bs-23029R-BF350 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Dnmt3a Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-23029R-BF350)?
KLH conjugated synthetic peptide derived from human DNMT-3 alpha
What are the excitation and emission wavelengths of the AbBy Fluor-350 conjugate (bs-23029R-BF350)?
AbBy Fluor-350 on bs-23029R-BF350: excitation / emission 346nm/442nm.