Help

NUCB2 Polyclonal Antibody, RBITC Conjugated

Applications

  • IF(ICC)
  • IF

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Dog
  • Cow
  • Pig
  • Horse
  • Rabbit
Overview
Catalog # bs-23043R-RBITC-100uL
Product Name NUCB2 Polyclonal Antibody, RBITC Conjugated
Applications
IF(ICC) IF
Reactivity Human, Mouse, Rat
Predicted Reactivity Dog, Cow, Pig, Horse, Rabbit
Specifications
Conjugation RBITC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Nucleobindin 2
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 4925
Swiss Prot P80303
Synonyms DNA binding protein NEFA; DNA-binding protein NEFA; Gastric cancer antigen Zg4; NEFA; Nucb2; NUCB2_HUMAN; Nucleobindin-2; Nucleobinding 2.
Background This gene encodes a protein with a suggested role in calcium level maintenance, eating regulation in the hypothalamus, and release of tumor necrosis factor from vascular endothelial cells. This protein binds calcium and has EF-folding domains. [provided by RefSeq, Oct 2011].
Application Dilution
IF(ICC) 1:50-200
IF

Frequently asked questions

Which applications is NUCB2 Polyclonal Antibody, RBITC Conjugated (bs-23043R-RBITC) validated for, and at what dilution?

bs-23043R-RBITC is validated for IF(ICC) (1:50-200); IF. These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does NUCB2 Polyclonal Antibody, RBITC Conjugated (bs-23043R-RBITC) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Pig, Horse, Rabbit.

How should bs-23043R-RBITC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise NUCB2 Polyclonal Antibody, RBITC Conjugated (bs-23043R-RBITC)?

KLH conjugated synthetic peptide derived from human Nucleobindin 2

What are the excitation and emission wavelengths of the RBITC conjugate (bs-23043R-RBITC)?

RBITC on bs-23043R-RBITC: excitation / emission 570nm/595nm.