Hes5 Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Human brain glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Hes5 Polyclonal Antibody, Unconjugated (bs-23071R) at 1:400 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human brain glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Hes5 Polyclonal Antibody, Unconjugated (bs-23071R) at 1:400 overnight at 4\u00b0C, DAB staining.

Applications

IHC-P
IHC-F
IF(ICC)
IF

Reactivity

Human
Mouse

Predicted Reactivity

Rat
Dog
Cow
Horse

Overview
Catalog #	bs-23071r
Product Name	Hes5 Polyclonal Antibody
Applications	IHC-P IHC-F IF(ICC) IF
Reactivity	Human, Mouse
Predicted Reactivity	Rat, Dog, Cow, Horse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Hes5
Immunogen Range	31-130/166
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	388585
Swiss Prot	Q5TA89
Synonyms	Hairy and enhancer of split 5; HES 5; HES5 protein; MGI:104876; HES5_HUMAN.
Background	This gene encodes a member of a family of basic helix-loop-helix transcriptional repressors. The protein product of this gene, which is activated downstream of the Notch pathway, regulates cell differentiation in multiple tissues. Disruptions in the normal expression of this gene have been associated with developmental diseases and cancer. [provided by RefSeq, Dec 2008]
Application Dilution
IHC-P
IHC-F
IF(ICC)
IF