connexin 30 Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-23115r-cy3-100uL |
| Product Name | connexin 30 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(ICC)
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human connexin |
| Immunogen Range | 201-261/261 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 10804 |
| Swiss Prot | O95452 |
| Synonyms | Connexin 30; Connexin-30; Cx30; CXB6_HUMAN; DFNA3; ectodermal dysplasia 2, hidrotic (Clouston syndrome); ED2; EDH; Gap junction beta 6 protein; Gap junction beta-6 protein; gap junction protein, beta 6 (connexin 30); gap junction protein, beta 6; GJB6; HED; NSRD1. |
| Background | The connexin family of proteins form hexameric complexes called connexons that facilitate movement of low molecular weight proteins between cells via gap junctions. Connexin proteins share a common topology of four transmembrane alpha-helical domains, two extracellular loops, a cytoplasmic loop and cytoplasmic N- and C-termini. Many of the key functional differences between connexins arise from specific amino-acid substitutions in the most highly conserved domains: the transmembrane and extracellular regions. Connexin 30, also known as GJB6 (Gap junction beta 6), ED2, EDH, HED or DFNA3, is a 261 amino acid multi-pass membrane protein that localizes to the cell junction and belongs to the connexin family. Functioning as a hexamer with other connexin proteins, connexin 30 facilitates the diffusion of low molecular weight materials from one cell to another. Defects in the gene encoding connexin 30 are the cause of ectodermal dysplasia type 2 (ED2) and non-syndromatic sensorineural deafness autosomal dominant type 3 (DFNA3), the former of which is characterized by abnormal development of ectodermal structures (such as skin and nails). |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(ICC) | 1:50-200 |
| IF | |
Frequently asked questions
Which applications is connexin 30 Polyclonal Antibody, Cy3 Conjugated (bs-23115R-Cy3) validated for, and at what dilution?
bs-23115R-Cy3 is validated for WB (1:300-5000); IF(ICC) (1:50-200); IF. These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does connexin 30 Polyclonal Antibody, Cy3 Conjugated (bs-23115R-Cy3) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-23115R-Cy3 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise connexin 30 Polyclonal Antibody, Cy3 Conjugated (bs-23115R-Cy3)?
KLH conjugated synthetic peptide derived from human connexin Immunogen range: 201-261/261.
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-23115R-Cy3)?
Cy3 on bs-23115R-Cy3: excitation / emission 512,550nm/570,615nm.