Myc tag Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-23166R-Cy3-100uL |
| Product Name | Myc tag Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Species independent |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated Myc tag(EQKLISEEDL) |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4609 |
| Swiss Prot | P01106 |
| Subcellular location | Nucleus |
| Synonyms | c-myc tag; Myc Epitope Tag. |
| Background | Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties. The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast,insect and mammalian cell systems. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Myc tag Antibody, Cy3 Conjugated (bs-23166R-Cy3) validated for, and at what dilution?
bs-23166R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Myc tag Antibody, Cy3 Conjugated (bs-23166R-Cy3) react with?
Tested reactivity: Species independent.
How should bs-23166R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Myc tag Antibody, Cy3 Conjugated (bs-23166R-Cy3)?
KLH conjugated Myc tag(EQKLISEEDL)
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-23166R-Cy3)?
Cy3 on bs-23166R-Cy3: excitation / emission 512,550nm/570,615nm.