KT3 tag Antibody, FITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-23288R-FITC-100uL |
| Product Name | KT3 tag Antibody, FITC Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Tags |
| Specifications | |
| Conjugation | FITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide of KT3 tag |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Synonyms | KPPTPPPEPET Epitope Tag; KPPTPPPEPET Tag; KT3 Epitope Tag. |
| Background | The KT3 epitope tag is commonly engineered onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized using immunochemical methods. The recognized KT3 epitope represents the amino acid sequence KPPTPPPEPET derived from the Simian Virus 40 (SV40) large T-antigen. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is KT3 tag Antibody, FITC Conjugated (bs-23288R-FITC) validated for, and at what dilution?
bs-23288R-FITC is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does KT3 tag Antibody, FITC Conjugated (bs-23288R-FITC) react with?
Tested reactivity: Tags.
How should bs-23288R-FITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise KT3 tag Antibody, FITC Conjugated (bs-23288R-FITC)?
KLH conjugated synthetic peptide of KT3 tag
What are the excitation and emission wavelengths of the FITC conjugate (bs-23288R-FITC)?
FITC on bs-23288R-FITC: excitation / emission 494nm/518nm.