B7-1/CD80 Polyclonal Antibody

Mouse thymus lysates probed with CD80 Polyclonal Antibody, Unconjugated (bs-23296R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

$Lane 1: Mouse Thymus lysates; Lane 2: Mouse RAW264.7 cell lysates; Lane 3: Mouse Sp2\/0 cell lysates; Lane 4: Rat Thymus cell lysates; Lane 5: Human Raji cell lysates; Lane 6: Human K562 cell lysates probed with CD80 Polyclonal Antibody, Unconjugated (bs-23296R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Thymus lysates; Lane 2: Mouse RAW264.7 cell lysates; Lane 3: Mouse Sp2\/0 cell lysates; Lane 4: Rat Thymus cell lysates; Lane 5: Human Raji cell lysates; Lane 6: Human K562 cell lysates probed with CD80 Polyclonal Antibody, Unconjugated (bs-23296R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA

Reactivity

Mouse
Rat

Overview
Catalog #	bs-23296R
Product Name	B7-1/CD80 Polyclonal Antibody
Applications	WB, ELISA
Reactivity	Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from mouse CD80
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	12519
Swiss Prot	Q00609
Subcellular location	Cell membrane
Synonyms	T-lymphocyte activation antigen CD80; Cd80; Activation B7-1 antigen; B7;
Background	CD80 is a member of the Ig superfamily and serves as the ligand for two T cell molecules, CD28 and CTLA4. Interactions between CD28 and CD80 on activated B cells result in enhanced T cell activation. CD80 is rapidly induced on the surface of in vitro activated B cells, Epstein Barr Virus (EBV) transformed B cell lines, Burkitts lymphoma cell lines, freshly isolated follicular B lymphoma cells, T cells, and monocytes. It is also expressed at high levels in dendritic cells. It reacts weakly with a small proportion of non activated normal B cells and with HTLV1 infected T cells. CD80 does not react with peripheral monocytes, resting and activated normal T cells, T cell lines and T cell clones, nor with myelomonocytic cell lines.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000