B7-1/CD80 Polyclonal Antibody, APC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-23296R-APC |
| Product Name | B7-1/CD80 Polyclonal Antibody, APC Conjugated |
| Applications |
WB
|
| Reactivity | Mouse, Rat |
| Specifications | |
| Conjugation | APC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from mouse CD80 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 12519 |
| Swiss Prot | Q00609 |
| Subcellular location | Cell membrane |
| Synonyms | T-lymphocyte activation antigen CD80; Cd80; Activation B7-1 antigen; B7; |
| Background | CD80 is a member of the Ig superfamily and serves as the ligand for two T cell molecules, CD28 and CTLA4. Interactions between CD28 and CD80 on activated B cells result in enhanced T cell activation. CD80 is rapidly induced on the surface of in vitro activated B cells, Epstein Barr Virus (EBV) transformed B cell lines, Burkitts lymphoma cell lines, freshly isolated follicular B lymphoma cells, T cells, and monocytes. It is also expressed at high levels in dendritic cells. It reacts weakly with a small proportion of non activated normal B cells and with HTLV1 infected T cells. CD80 does not react with peripheral monocytes, resting and activated normal T cells, T cell lines and T cell clones, nor with myelomonocytic cell lines. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is B7-1/CD80 Polyclonal Antibody, APC Conjugated (bs-23296R-APC) validated for, and at what dilution?
bs-23296R-APC is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does B7-1/CD80 Polyclonal Antibody, APC Conjugated (bs-23296R-APC) react with?
Tested reactivity: Mouse, Rat.
How should bs-23296R-APC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise B7-1/CD80 Polyclonal Antibody, APC Conjugated (bs-23296R-APC)?
KLH conjugated synthetic peptide derived from mouse CD80
What are the excitation and emission wavelengths of the APC conjugate (bs-23296R-APC)?
APC on bs-23296R-APC: excitation / emission 650nm/660nm.