PKC epsilon Polyclonal Antibody, PE-Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-2329R-PE-Cy5 |
| Product Name | PKC epsilon Polyclonal Antibody, PE-Cy5 Conjugated |
| Applications |
WB
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Cow, Pig, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | PE-Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human PKCE (AGC-kinase C-terminal) |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5581 |
| Swiss Prot | Q02156 |
| Subcellular location | Cytoplasm, Nucleus, Cell membrane |
| Synonyms | PKCE; nPKC-epsilon; Protein kinase C epsilon type; PRKCE |
| Background | Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been shown to be involved in many different cellular functions, such as neuron channel activation, apoptosis, cardioprotection from ischemia, heat shock response, as well as insulin exocytosis. Knockout studies in mice suggest that this kinase is important for lipopolysaccharide (LPS)-mediated signaling in activated macrophages and may also play a role in controlling anxiety-like behavior. [provided by RefSeq, Jul 2008] |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is PKC epsilon Polyclonal Antibody, PE-Cy5 Conjugated (bs-2329R-PE-Cy5) validated for, and at what dilution?
bs-2329R-PE-Cy5 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PKC epsilon Polyclonal Antibody, PE-Cy5 Conjugated (bs-2329R-PE-Cy5) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Pig, Horse, Chicken, Rabbit.
How should bs-2329R-PE-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PKC epsilon Polyclonal Antibody, PE-Cy5 Conjugated (bs-2329R-PE-Cy5)?
KLH conjugated synthetic peptide derived from human PKCE (AGC-kinase C-terminal)
What are the excitation and emission wavelengths of the PE-Cy5 conjugate (bs-2329R-PE-Cy5)?
PE-Cy5 on bs-2329R-PE-Cy5: excitation / emission 488nm/670nm.