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RAP2B Polyclonal Antibody, AbBy Fluor-750 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Predicted Reactivity

  • Human
  • Mouse
  • Rat
  • Dog
  • Cow
  • Sheep
  • Horse
Overview
Catalog # bs-2330R-BF750
Product Name RAP2B Polyclonal Antibody, AbBy Fluor-750 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Predicted Reactivity Human, Mouse, Rat, Dog, Cow, Sheep, Horse
Specifications
Conjugation AbBy Fluor-750
Host Rabbit
Source KLH conjugated synthetic peptide derived from human RAP2B
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 5912
Subcellular location Cytoplasm, Cell membrane
Synonyms RAP 2B; RAP2B member of RAS oncogene family; Ras family small GTP binding protein RAP2B; Ras related protein RAP 2B; Ras related protein RAP2B ; Small GTP binding protein.RAP2B_HUMAN
Background RAP2B belongs to a family of RAS-related GTP-binding proteins. RAP proteins share approximately 50% amino acid identity with the classical RAS proteins and have numerous structural features in common. However, at their 61st amino acid the glutamine in RAS proteins is replaced by threonine in RAP proteins. RAP2A interacts with phospholipase C, epsilon 1 (PLCE1).
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is RAP2B Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-2330R-BF750) validated for, and at what dilution?

bs-2330R-BF750 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

How should bs-2330R-BF750 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise RAP2B Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-2330R-BF750)?

KLH conjugated synthetic peptide derived from human RAP2B

What are the excitation and emission wavelengths of the AbBy Fluor-750 conjugate (bs-2330R-BF750)?

AbBy Fluor-750 on bs-2330R-BF750: excitation / emission 749nm/775nm.