DEPTOR Polyclonal Antibody, PE-Cy7 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-23314r-pe-cy7-100uL |
| Product Name | DEPTOR Polyclonal Antibody, PE-Cy7 Conjugated |
| Applications |
WB
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | PE-Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human DEPTOR |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | Q8TB45 |
| Swiss Prot | 64798 |
| Synonyms | DEP domain containing 6; DEP domain-containing mTOR-interacting protein; DEP domain-containing protein 6; DEPDC6; DEPTOR; DPTOR_HUMAN. |
| Background | DEPTOR (DEP domain containing MTOR-interacting protein), also known as DEP.6 or DEPDC6 (DEP domain-containing protein 6), is a 409 amino acid protein that negatively regulates mTORC1 and mTORC2 pathways. DEPTOR interacts with FRAP via its PDZ domain, and undergoes post-translational phosphorylation. Containing two DEP domains and one PDZ (DHR) domain, DEPTOR is encoded by a gene that maps to human chromosome 8q24.12. Chromosome 8 consists of nearly 146 million base pairs, encodes over 800 genes and is associated with a variety of diseases and malignancies. Schizophrenia, bipolar disorder, Trisomy 8, Pfeiffer syndrome, congenital hypothyroidism, Waardenburg syndrome and some leukemias and lymphomas are thought to occur as a result of defects in specific genes that map to chromosome 8. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is DEPTOR Polyclonal Antibody, PE-Cy7 Conjugated (bs-23314R-PE-Cy7) validated for, and at what dilution?
bs-23314R-PE-Cy7 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does DEPTOR Polyclonal Antibody, PE-Cy7 Conjugated (bs-23314R-PE-Cy7) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-23314R-PE-Cy7 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise DEPTOR Polyclonal Antibody, PE-Cy7 Conjugated (bs-23314R-PE-Cy7)?
KLH conjugated synthetic peptide derived from human DEPTOR
What are the excitation and emission wavelengths of the PE-Cy7 conjugate (bs-23314R-PE-Cy7)?
PE-Cy7 on bs-23314R-PE-Cy7: excitation / emission 488nm/780nm.