SMAD6 Polyclonal Antibody, AbBy Fluor-350 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-23329R-BF350-100uL |
| Product Name | SMAD6 Polyclonal Antibody, AbBy Fluor-350 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-350 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human SMAD6 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4091 |
| Swiss Prot | O43541 |
| Synonyms | SMAD Family Member 6; MAD Homolog 6; MADH6; MAD, Mothers Against Decapentaplegic Homolog 6 (Drosophila) ; Mothers Against Decapentaplegic, Drosophila, Homolog Of, 6; SMAD, Mothers Against DPP Homolog 6 (Drosophila) ; Mothers Against Decapentaplegic Homolog 6; SMAD, Mothers Against DPP Homolog 6; Mothers Against DPP Homolog 6; HsT17432; SMAD 6; HSMAD6; AOVD2; MADH7; SMAD6_HUMAN; |
| Background | The protein encoded by this gene belongs to the SMAD family of proteins, which are related to Drosophila 'mothers against decapentaplegic' (Mad) and C. elegans Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions in the negative regulation of BMP and TGF-beta/activin-signalling. Multiple transcript variants have been found for this gene.[provided by RefSeq, Sep 2014] |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is SMAD6 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-23329R-BF350) validated for, and at what dilution?
bs-23329R-BF350 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does SMAD6 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-23329R-BF350) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit.
How should bs-23329R-BF350 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise SMAD6 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-23329R-BF350)?
KLH conjugated synthetic peptide derived from human SMAD6
What are the excitation and emission wavelengths of the AbBy Fluor-350 conjugate (bs-23329R-BF350)?
AbBy Fluor-350 on bs-23329R-BF350: excitation / emission 346nm/442nm.