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SMAD6 Polyclonal Antibody, Biotin Conjugated

Applications

  • WB
  • IHC-P
  • IHC-F

Reactivity

  • Mouse

Predicted Reactivity

  • Human
  • Rat
  • Dog
  • Cow
  • Sheep
  • Pig
  • Horse
  • Chicken
  • Rabbit
Overview
Catalog # bs-23329R-Biotin-100uL
Product Name SMAD6 Polyclonal Antibody, Biotin Conjugated
Applications
WB IHC-P IHC-F
Reactivity Mouse
Predicted Reactivity Human, Rat, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit
Specifications
Conjugation Biotin
Host Rabbit
Source KLH conjugated synthetic peptide derived from human SMAD6
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C for 12 months.
Target
Gene ID 4091
Swiss Prot O43541
Synonyms SMAD Family Member 6; MAD Homolog 6; MADH6; MAD, Mothers Against Decapentaplegic Homolog 6 (Drosophila) ; Mothers Against Decapentaplegic, Drosophila, Homolog Of, 6; SMAD, Mothers Against DPP Homolog 6 (Drosophila) ; Mothers Against Decapentaplegic Homolog 6; SMAD, Mothers Against DPP Homolog 6; Mothers Against DPP Homolog 6; HsT17432; SMAD 6; HSMAD6; AOVD2; MADH7; SMAD6_HUMAN;
Background The protein encoded by this gene belongs to the SMAD family of proteins, which are related to Drosophila 'mothers against decapentaplegic' (Mad) and C. elegans Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions in the negative regulation of BMP and TGF-beta/activin-signalling. Multiple transcript variants have been found for this gene.[provided by RefSeq, Sep 2014]
Application Dilution
WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Frequently asked questions

Which applications is SMAD6 Polyclonal Antibody, Biotin Conjugated (bs-23329R-Biotin) validated for, and at what dilution?

bs-23329R-Biotin is validated for WB (1:300-5000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does SMAD6 Polyclonal Antibody, Biotin Conjugated (bs-23329R-Biotin) react with?

Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit.

How should bs-23329R-Biotin be stored?

Store at -20C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise SMAD6 Polyclonal Antibody, Biotin Conjugated (bs-23329R-Biotin)?

KLH conjugated synthetic peptide derived from human SMAD6