DGAT1 Polyclonal Antibody, AbBy Fluor-647 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-2332R-BF647 |
| Product Name | DGAT1 Polyclonal Antibody, AbBy Fluor-647 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Dog, Pig, Horse |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human DGAT1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 8694 |
| Swiss Prot | O75907 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | ARAT; DGAT; ARGP1; DIAR7; Diacylglycerol O-acyltransferase 1; ACAT-related gene product 1; Acyl-CoA retinol O-fatty-acyltransferase; Retinol O-fatty-acyltransferase; Diglyceride acyltransferase; DGAT1; AGRP1 |
| Background | Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. In contrast to DGAT2 it is not essential for survival. May be involved in VLDL (very low density lipoprotein) assembly. In liver, plays a role in esterifying exogenous fatty acids to glycerol. Functions as the major acyl-CoA retinol acyltransferase (ARAT) in the skin, where it acts to maintain retinoid homeostasis and prevent retinoid toxicity leading to skin and hair disorders. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is DGAT1 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-2332R-BF647) validated for, and at what dilution?
bs-2332R-BF647 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does DGAT1 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-2332R-BF647) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Pig, Horse.
How should bs-2332R-BF647 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise DGAT1 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-2332R-BF647)?
KLH conjugated synthetic peptide derived from human DGAT1
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bs-2332R-BF647)?
AbBy Fluor-647 on bs-2332R-BF647: excitation / emission 650nm/665nm.