Help

IRS-4 Polyclonal Antibody, PE-Cy7 Conjugated

Applications

  • WB

Reactivity

  • Human
  • Mouse

Predicted Reactivity

  • Rat
Overview
Catalog # bs-23362R-PE-Cy7-100uL
Product Name IRS-4 Polyclonal Antibody, PE-Cy7 Conjugated
Applications
WB
Reactivity Human, Mouse
Predicted Reactivity Rat
Specifications
Conjugation PE-Cy7
Host Rabbit
Source KLH conjugated synthetic peptide derived from human IRS-4
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 8471
Swiss Prot O14654
Subcellular location Cytoplasm, Cell membrane
Synonyms IRS4; 160 kDa phosphotyrosine protein; Insulin receptor substrate 4; IRS 4; PY160; IRS4_HUMAN; IRS-4; Phosphoprotein of 160 kDa; pp160.
Background IRS4 (Insulin receptor substrate 4) is a cytoplasmic protein that contains many potential tyrosine and serine/threonine phosphorylation sites. Tyrosine-phosphorylated IRS4 protein has been shown to associate with cytoplasmic signaling molecules that contain SH2 domains. The IRS4 protein is phosphorylated by the insulin receptor tyrosine kinase upon receptor stimulation.
Application Dilution
WB 1:300-5000

Frequently asked questions

Which applications is IRS-4 Polyclonal Antibody, PE-Cy7 Conjugated (bs-23362R-PE-Cy7) validated for, and at what dilution?

bs-23362R-PE-Cy7 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does IRS-4 Polyclonal Antibody, PE-Cy7 Conjugated (bs-23362R-PE-Cy7) react with?

Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat.

How should bs-23362R-PE-Cy7 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise IRS-4 Polyclonal Antibody, PE-Cy7 Conjugated (bs-23362R-PE-Cy7)?

KLH conjugated synthetic peptide derived from human IRS-4

What are the excitation and emission wavelengths of the PE-Cy7 conjugate (bs-23362R-PE-Cy7)?

PE-Cy7 on bs-23362R-PE-Cy7: excitation / emission 488nm/780nm.