IRS-4 Polyclonal Antibody, PerCP Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-23362R-PerCP-100uL |
| Product Name | IRS-4 Polyclonal Antibody, PerCP Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat |
| Specifications | |
| Conjugation | PerCP |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human IRS-4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 8471 |
| Swiss Prot | O14654 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | IRS4; 160 kDa phosphotyrosine protein; Insulin receptor substrate 4; IRS 4; PY160; IRS4_HUMAN; IRS-4; Phosphoprotein of 160 kDa; pp160. |
| Background | IRS4 (Insulin receptor substrate 4) is a cytoplasmic protein that contains many potential tyrosine and serine/threonine phosphorylation sites. Tyrosine-phosphorylated IRS4 protein has been shown to associate with cytoplasmic signaling molecules that contain SH2 domains. The IRS4 protein is phosphorylated by the insulin receptor tyrosine kinase upon receptor stimulation. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is IRS-4 Polyclonal Antibody, PerCP Conjugated (bs-23362R-PerCP) validated for, and at what dilution?
bs-23362R-PerCP is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does IRS-4 Polyclonal Antibody, PerCP Conjugated (bs-23362R-PerCP) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat.
How should bs-23362R-PerCP be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise IRS-4 Polyclonal Antibody, PerCP Conjugated (bs-23362R-PerCP)?
KLH conjugated synthetic peptide derived from human IRS-4
What are the excitation and emission wavelengths of the PerCP conjugate (bs-23362R-PerCP)?
PerCP on bs-23362R-PerCP: excitation / emission 482nm/677nm.