phospho-C-Myc (Thr58) Polyclonal Antibody, PE-Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-23391R-PE-Cy7-100uL |
| Product Name | phospho-C-Myc (Thr58) Polyclonal Antibody, PE-Cy7 Conjugated |
| Applications |
WB
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Dog, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | PE-Cy7 |
| Host | Rabbit |
| Source | KLH conjugated Synthesised phosphopeptide derived from human C-Myc around the phosphorylation site of Thr58 |
| Modification Site | Thr58 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4609 |
| Swiss Prot | P01106 |
| Subcellular location | Nucleus |
| Synonyms | Myc(Phospho-Thr58); Myc(Phospho-T58); p-Myc(Thr58); p-Myc(T58); AU016757; Avian myelocytomatosis viral oncogene homolog; bHLHe39; c Myc; Cellular myelocytomatosis oncogene; MGC105490; MRTL; Myc protein; Myc proto oncogene protein; Myc-related translation/localization regulatory factor; Myc2; myca; Myelocytomatosis oncogene a; Myelocytomatosis oncogene; Niard; Nird; Oncogene Myc; Protooncogene homologous to myelocytomatosis virus; RNCMYC; Transcription factor p64; Transcriptional regulator Myc-A; v myc avian myelocytomatosis viral oncogene homolog; v myc myelocytomatosis viral oncogene homolog (avian); V-Myc avian myelocytomatosis viral oncogene homolog; v-myc myelocytomatosis viral oncogene homolog (avian); zc-myc; MYC_HUMAN. |
| Background | The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene. [provided by RefSeq, Jul 2008]. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is phospho-C-Myc (Thr58) Polyclonal Antibody, PE-Cy7 Conjugated (bs-23391R-PE-Cy7) validated for, and at what dilution?
bs-23391R-PE-Cy7 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-C-Myc (Thr58) Polyclonal Antibody, PE-Cy7 Conjugated (bs-23391R-PE-Cy7) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Pig, Horse, Rabbit.
How should bs-23391R-PE-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-C-Myc (Thr58) Polyclonal Antibody, PE-Cy7 Conjugated (bs-23391R-PE-Cy7)?
KLH conjugated Synthesised phosphopeptide derived from human C-Myc around the phosphorylation site of Thr58
What are the excitation and emission wavelengths of the PE-Cy7 conjugate (bs-23391R-PE-Cy7)?
PE-Cy7 on bs-23391R-PE-Cy7: excitation / emission 488nm/780nm.