SOD2 Polyclonal Antibody, AbBy Fluor-647 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-23402R-BF647 |
| Product Name | SOD2 Polyclonal Antibody, AbBy Fluor-647 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Horse, Chicken |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human SOD2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 6648 |
| Swiss Prot | P04179 |
| Subcellular location | Cytoplasm |
| Synonyms | IPO B; Manganese SOD; Superoxide Dismutase 2; Manganese superoxide dismutase; Mn SOD; MNSOD; SOD 2; SOD-2; SOD2; Superoxide dismutase [Mn] mitochondrial; Superoxide dismutase [Mn] mitochondrial precursor; Superoxide dismutase 2 mitochondrial; SODM_HUMAN. |
| Background | This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008] |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is SOD2 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-23402R-BF647) validated for, and at what dilution?
bs-23402R-BF647 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does SOD2 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-23402R-BF647) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Horse, Chicken.
How should bs-23402R-BF647 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise SOD2 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-23402R-BF647)?
KLH conjugated synthetic peptide derived from human SOD2
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bs-23402R-BF647)?
AbBy Fluor-647 on bs-23402R-BF647: excitation / emission 650nm/665nm.