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ASNS Polyclonal Antibody, RBITC Conjugated

Applications

  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Dog
  • Cow
  • Pig
  • Horse
  • Rabbit
Overview
Catalog # bs-23431R-RBITC-100uL
Product Name ASNS Polyclonal Antibody, RBITC Conjugated
Applications
IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse, Rat
Predicted Reactivity Dog, Cow, Pig, Horse, Rabbit
Specifications
Conjugation RBITC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Asparagine synthetase
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 440
Swiss Prot P08243
Subcellular location Cytoplasm
Synonyms asnS; ASNS_HUMAN; ASNSD; Asparagine synthetase [glutamine-hydrolyzing]; Cell cycle control protein TS11; Glutamine dependent asparagine synthetase 3; Glutamine dependent asparagine synthetase; Glutamine hydrolyzing; Glutamine-dependent asparagine synthetase; OTTHUMP00000024510; OTTHUMP00000204938; OTTHUMP00000204939; OTTHUMP00000204940; OTTHUMP00000204941; OTTHUMP00000204942; TS11; TS11 cell cycle control protein.
Background The protein encoded by this gene is involved in the synthesis of asparagine. This gene complements a mutation in the temperature-sensitive hamster mutant ts11, which blocks progression through the G1 phase of the cell cycle at nonpermissive temperature. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010]
Application Dilution
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is ASNS Polyclonal Antibody, RBITC Conjugated (bs-23431R-RBITC) validated for, and at what dilution?

bs-23431R-RBITC is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does ASNS Polyclonal Antibody, RBITC Conjugated (bs-23431R-RBITC) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Pig, Horse, Rabbit.

How should bs-23431R-RBITC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise ASNS Polyclonal Antibody, RBITC Conjugated (bs-23431R-RBITC)?

KLH conjugated synthetic peptide derived from human Asparagine synthetase

What are the excitation and emission wavelengths of the RBITC conjugate (bs-23431R-RBITC)?

RBITC on bs-23431R-RBITC: excitation / emission 540nm/625nm.