IL-4R Polyclonal Antibody, AbBy Fluor-647 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-23578R-BF647-100uL |
| Product Name | IL-4R Polyclonal Antibody, AbBy Fluor-647 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human IL-4R |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3566 |
| Swiss Prot | P24394 |
| Subcellular location | Secreted, Cell membrane |
| Synonyms | Interleukin-4 Receptor; 582J2.1; CD124; CD-124; CD 124; IL 4R alpha; IL4RA; Interleukin 4 receptor alpha chain; MGC118473. |
| Background | This gene encodes the alpha chain of the interleukin-4 receptor, a type I transmembrane protein that can bind interleukin4 and interleukin 13 to regulate IgE production. The encoded protein also can bind interleukin 4 to promote differentiation of Th2 cells. A soluble form of the encoded protein can be produced by an alternate splice variant or by proteolysis of the membrane-bound protein, and this soluble form can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells. Allelic variations in this gene have been associated with atopy, a condition that can manifest itself as allergic rhinitis, sinusitus, asthma, or eczema. Two transcript variants encoding different isoforms, a membrane-bound and a soluble form, have been found for this gene. [provided by RefSeq, Jul 2008]. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is IL-4R Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-23578R-BF647) validated for, and at what dilution?
bs-23578R-BF647 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does IL-4R Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-23578R-BF647) react with?
Tested reactivity: Human.
How should bs-23578R-BF647 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise IL-4R Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-23578R-BF647)?
KLH conjugated synthetic peptide derived from human IL-4R
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bs-23578R-BF647)?
AbBy Fluor-647 on bs-23578R-BF647: excitation / emission 650nm/665nm.