Wnt1 Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-23583R-Cy5.5-100uL |
| Product Name | Wnt1 Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Wnt1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 7471 |
| Swiss Prot | P04628 |
| Subcellular location | Secreted, Extracellular matrix |
| Synonyms | wingless-type MMTV integration site family, member 1; INT 1; INT1; Murine mammary tumor virus integration site 1 ; Oncogene INT1; Proto oncogene protein Wnt 1; Wnt 1; Wnt1 proto oncogene protein precursor; WNT1_HUMAN. |
| Background | Ligand for members of the frizzled family of seven transmembrane receptors. In some developmental processes, is also a ligand for the coreceptor RYK, thus triggering Wnt signaling. Probable developmental protein. May be a signaling molecule important in CNS development. Is likely to signal over only few cell diameters. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Wnt1 Polyclonal Antibody, Cy5.5 Conjugated (BS-23583R-CY5-5) validated for, and at what dilution?
BS-23583R-CY5-5 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Wnt1 Polyclonal Antibody, Cy5.5 Conjugated (BS-23583R-CY5-5) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Pig, Horse, Rabbit.
How should BS-23583R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Wnt1 Polyclonal Antibody, Cy5.5 Conjugated (BS-23583R-CY5-5)?
KLH conjugated synthetic peptide derived from human Wnt1
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-23583R-CY5-5)?
Cy5.5 on BS-23583R-CY5-5: excitation / emission 675nm/694nm.