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Wnt1 Polyclonal Antibody, FITC Conjugated

Applications

  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse

Predicted Reactivity

  • Pig
  • Horse
  • Rabbit
Overview
Catalog # bs-23583R-FITC-100uL
Product Name Wnt1 Polyclonal Antibody, FITC Conjugated
Applications
IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse
Predicted Reactivity Pig, Horse, Rabbit
Specifications
Conjugation FITC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Wnt1
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 7471
Swiss Prot P04628
Subcellular location Secreted, Extracellular matrix
Synonyms wingless-type MMTV integration site family, member 1; INT 1; INT1; Murine mammary tumor virus integration site 1 ; Oncogene INT1; Proto oncogene protein Wnt 1; Wnt 1; Wnt1 proto oncogene protein precursor; WNT1_HUMAN.
Background Ligand for members of the frizzled family of seven transmembrane receptors. In some developmental processes, is also a ligand for the coreceptor RYK, thus triggering Wnt signaling. Probable developmental protein. May be a signaling molecule important in CNS development. Is likely to signal over only few cell diameters.
Application Dilution
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is Wnt1 Polyclonal Antibody, FITC Conjugated (bs-23583R-FITC) validated for, and at what dilution?

bs-23583R-FITC is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Wnt1 Polyclonal Antibody, FITC Conjugated (bs-23583R-FITC) react with?

Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Pig, Horse, Rabbit.

How should bs-23583R-FITC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Wnt1 Polyclonal Antibody, FITC Conjugated (bs-23583R-FITC)?

KLH conjugated synthetic peptide derived from human Wnt1

What are the excitation and emission wavelengths of the FITC conjugate (bs-23583R-FITC)?

FITC on bs-23583R-FITC: excitation / emission 494nm/518nm.

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