UBF1 Polyclonal Antibody

$Formalin-fixed and paraffin embedded human endometrial carcinoma labeled with Anti-UBF1\/RNA polymerase I Polyclonal Antibody, Unconjugated (bs-2362R) at 1:200 followed by conjugation to the secondary antibody and DAB staining$

Formalin-fixed and paraffin embedded human endometrial carcinoma labeled with Anti-UBF1\/RNA polymerase I Polyclonal Antibody, Unconjugated (bs-2362R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

$Lane 1: Mouse Cerebrum lysates; Lane 2: Human U-2OS\r\n cell lysates; Lane 3: Human MOLT-4 cell lysates probed with UBF1 Polyclonal Antibody, Unconjugated (bs-2362R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Cerebrum lysates; Lane 2: Human U-2OS\r\n cell lysates; Lane 3: Human MOLT-4 cell lysates probed with UBF1 Polyclonal Antibody, Unconjugated (bs-2362R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse

Predicted Reactivity

Overview
Catalog #	bs-2362R
Product Name	UBF1 Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human, Mouse
Predicted Reactivity	Rat, Cow
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human UBF1
Immunogen Range	51-150/764
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	7343
Swiss Prot	P17480
Subcellular location	Nucleus
Synonyms	UBF; UBF1; UBF2; UBF-1; NOR-90; Nucleolar transcription factor 1; Autoantigen NOR-90; Upstream-binding factor 1; UBTF
Background	Recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I through cooperative interactions with the transcription factor SL1/TIF-IB complex. It binds specifically to the upstream control element.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200

Applications

Reactivity

Predicted Reactivity

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