PITX3 Polyclonal Antibody, APC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-2364R-APC |
| Product Name | PITX3 Polyclonal Antibody, APC Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat, Cow |
| Predicted Reactivity | Dog, Pig, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | APC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human PITX3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5309 |
| Subcellular location | Nucleus |
| Synonyms | Homeobox protein PITX 3; Homeobox protein PITX3; MGC12766; Paired like homeodomain transcription factor 3; Paired-like homeodomain transcription factor 3; Pituitary homeobox 3; PITX 3; Pitx3; PITX3_HUMAN; PTX 3; PTX3. |
| Background | The transcription factor PITX3 is expressed selectively in the midbrain and regulates the differentiation and survival of dopaminergic neurons. Lack of this factor results in a degeneration similar to that seen in Parkinson's disease. PITX3 is also important in eye developement; mutations of the PITX3 gene have been associated with a familial form of cataracts. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is PITX3 Polyclonal Antibody, APC Conjugated (bs-2364R-APC) validated for, and at what dilution?
bs-2364R-APC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PITX3 Polyclonal Antibody, APC Conjugated (bs-2364R-APC) react with?
Tested reactivity: Human, Mouse, Rat, Cow. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Pig, Horse, Chicken, Rabbit.
How should bs-2364R-APC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PITX3 Polyclonal Antibody, APC Conjugated (bs-2364R-APC)?
KLH conjugated synthetic peptide derived from human PITX3
What are the excitation and emission wavelengths of the APC conjugate (bs-2364R-APC)?
APC on bs-2364R-APC: excitation / emission 650nm/660nm.