HSD3B7 Polyclonal Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-2366R |
| Product Name | HSD3B7 Polyclonal Antibody |
| Applications |
ELISA
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
IHC-P
|
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse, Cow, Pig, Rabbit |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human HSD3B7 |
| Immunogen Range | 281-369/369 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 80270 |
| Swiss Prot | Q9H2F3 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | 3 beta-hydroxysteroid dehydrogenase type 7; 3 beta-hydroxysteroid dehydrogenase type VII; 3-beta-HSD VII; 3-beta-hydroxy-Delta(5)-C27 steroid oxidoreductase; C(27) 3-beta-HSD; Cholest-5-ene-3-beta,7-alpha-diol 3-beta-dehydrogenase; HSD3B7 |
| Background | The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids. HSD VII is active against four 7-alpha-hydroxylated sterols. Does not metabolize several different C(19/21) steroids as substrates. Involved in bile acid synthesis. |
| Application Dilution | |
| ELISA | 1:500-1000 |
| IHC-F | 1:100-500 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
| IHC-P | 1:200-400 |
Frequently asked questions
Which applications is HSD3B7 Polyclonal Antibody (bs-2366R) validated for, and at what dilution?
bs-2366R is validated for ELISA (1:500-1000); IHC-F (1:100-500); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200); IHC-P (1:200-400). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does HSD3B7 Polyclonal Antibody (bs-2366R) react with?
Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Cow, Pig, Rabbit.
How should bs-2366R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise HSD3B7 Polyclonal Antibody (bs-2366R)?
KLH conjugated synthetic peptide derived from human HSD3B7 Immunogen range: 281-369/369.