PKC alpha Polyclonal Antibody, FITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-23716R-FITC |
| Product Name | PKC alpha Polyclonal Antibody, FITC Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | FITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human PKC alpha |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | P17252 |
| Swiss Prot | 5578 |
| Synonyms | AAG6; Aging associated gene 6; KPCA_HUMAN; MGC129900; MGC129901; PICK 1; PKC A; PKC alpha; PKC alpha; PKC-A; PKC-alpha; PKCA; PRKACA; PRKC A; PRKCA; Protein Kinase C alpha; Protein kinase C alpha type. |
| Background | Protein Kinase c alpha (PKC alpha) is an 77 kDa member of the conventional group (cPKCs: sensitive to calcium, diacylglycerol, phosphatidylserine and phorbol esters) of the PKC family of serine/ threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival and transcriptional regulation. PKC alpha is an ubiquitously expressed PKC isozyme that has been implicated in the regulation of a broad range of cellular functions including proliferation, differentiation, development, migration, cell cell adhesion, cell extracellular matrix adhesion, and solute transport. The activation loop threonine (threonine 497 in PKC alpha) of conventional PKCs is phosphorylated by phosphoinositide dependent kinase 1 (PDK1). This phosphorylation is necessary for the autophosphorylation of threonine 638 in the carboxy terminus of PKC alpha, a step that is critical for regulating the rate of PKC alpha dephosphorylation and inactivation. |
| Application Dilution | |
| WB | |
| IF | |
Frequently asked questions
Which applications is PKC alpha Polyclonal Antibody, FITC Conjugated (bs-23716R-FITC) validated for, and at what dilution?
bs-23716R-FITC is validated for WB.
Which species does PKC alpha Polyclonal Antibody, FITC Conjugated (bs-23716R-FITC) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-23716R-FITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PKC alpha Polyclonal Antibody, FITC Conjugated (bs-23716R-FITC)?
KLH conjugated synthetic peptide derived from human PKC alpha
What are the excitation and emission wavelengths of the FITC conjugate (bs-23716R-FITC)?
FITC on bs-23716R-FITC: excitation / emission 494nm/518nm.