LAMP2 Polyclonal Antibody

$Formalin-fixed and paraffin embedded mouse lung labeled with Anti-LAMP2\/CD107B Polyclonal Antibody, Unconjugated (bs-2379R) at 1:200 followed by conjugation to the secondary antibody and DAB staining$

Formalin-fixed and paraffin embedded mouse lung labeled with Anti-LAMP2\/CD107B Polyclonal Antibody, Unconjugated (bs-2379R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

$Mouse placenta lysates probed with LAMP2 Polyclonal Antibody, Unconjugated (bs-2379R) at 1:300 overnight at 4\u02daC. Followed by a conjugated secondary antibody at 1:10000 for 90 min at 37\u02daC$

Mouse placenta lysates probed with LAMP2 Polyclonal Antibody, Unconjugated (bs-2379R) at 1:300 overnight at 4\u02daC. Followed by a conjugated secondary antibody at 1:10000 for 90 min at 37\u02daC

Human U937 cells probed with LAMP2 Polyclonal Antibody, Unconjugated (bs-2379R) at 1:100 for 30 minutes followed by incubation with a PE Conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

$Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with LAMP2 Polyclonal Antibody, Unconjugated (bs-2379R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with LAMP2 Polyclonal Antibody, Unconjugated (bs-2379R) at 1:200 overnight at 4\u00b0C, DAB staining.

Applications

WB
FCM
IHC-P
IF(IHC-P)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-2379R
Product Name	LAMP2 Polyclonal Antibody
Applications	WB, FCM, IHC-P, IF(IHC-P)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human LAMP2
Immunogen Range	305-355/410
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage	Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for 12 months.
Target
Gene ID	3920
Swiss Prot	P13473
Subcellular location	Lumenal
Synonyms	LAMPB; CD107b; LAMP-2; LGP110; Lysosome-associated membrane glycoprotein 2; Lysosome-associated membrane protein 2; CD107 antigen-like family member B; LAMP2
Background	Implicated in tumor cell metastasis. May function in protection of the lysosomal membrane from autodigestion, maintenance of the acidic environment of the lysosome, adhesion when expressed on the cell surface (plasma membrane), and inter- and intracellular signal transduction. Protects cells from the toxic effects of methylating mutagens.
Application Dilution
WB	1:300-1000
FCM	1:20-100
IHC-P	1:200-400
IF(IHC-P)	1:50-200

Applications

Reactivity

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