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MyoD1/Myf3 Polyclonal Antibody, RBITC Conjugated

Applications

  • WB

Reactivity

  • Mouse
  • Rat

Predicted Reactivity

  • Human
  • Dog
  • Cow
  • Sheep
  • Pig
  • Horse
Overview
Catalog # bs-23809R-RBITC
Product Name MyoD1/Myf3 Polyclonal Antibody, RBITC Conjugated
Applications
WB
Reactivity Mouse, Rat
Predicted Reactivity Human, Dog, Cow, Sheep, Pig, Horse
Specifications
Conjugation RBITC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human MyoD1/Myf3:1-100/320
Clonality Polyclonal
Isotype IgG
Concentration 1mg/ml
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 4654
Swiss Prot P15172
Subcellular location Nucleus
Synonyms Myogenic differentiation 1; AI503393; bHLHc1; MD1; MGC156574; MYF3; MYOD; MYOD1; PUM; MYOD1_HUMAN; Class C basic helix-loop-helix protein 1; bHLHc1; Myogenic factor 3; Myf-3
Background Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Activates muscle-specific promoters. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins.

Application Dilution
WB 1:300-5000

Frequently asked questions

Which applications is MyoD1/Myf3 Polyclonal Antibody, RBITC Conjugated (bs-23809R-RBITC) validated for, and at what dilution?

bs-23809R-RBITC is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does MyoD1/Myf3 Polyclonal Antibody, RBITC Conjugated (bs-23809R-RBITC) react with?

Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Cow, Sheep, Pig, Horse.

How should bs-23809R-RBITC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise MyoD1/Myf3 Polyclonal Antibody, RBITC Conjugated (bs-23809R-RBITC)?

KLH conjugated synthetic peptide derived from human MyoD1/Myf3:1-100/320

What are the excitation and emission wavelengths of the RBITC conjugate (bs-23809R-RBITC)?

RBITC on bs-23809R-RBITC: excitation / emission 540nm/625nm.