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Histone H4 Polyclonal Antibody, AbBy Fluor-750 Conjugated

Applications

  • WB
  • IF

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bs-23810R-BF750-100uL
Product Name Histone H4 Polyclonal Antibody, AbBy Fluor-750 Conjugated
Applications
WB IF
Reactivity Human, Mouse, Rat
Specifications
Conjugation AbBy Fluor-750
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Histone H4
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 121504
Swiss Prot P62805
Synonyms H4F2; H4FN; HIST1H4; HIST2H4; HISTH4H4; methyl histone H4; histone H4; H4_HUMAN; Osteogenic growth peptide; OGP.
Background Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. [provided by RefSeq, Jul 2008].
Application Dilution
WB 1:500-2000
IF FCM1:100

Frequently asked questions

Which applications is Histone H4 Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-23810R-BF750) validated for, and at what dilution?

bs-23810R-BF750 is validated for WB (1:500-2000); FCM (1:100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Histone H4 Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-23810R-BF750) react with?

Tested reactivity: Human, Mouse, Rat.

How should bs-23810R-BF750 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Histone H4 Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-23810R-BF750)?

KLH conjugated synthetic peptide derived from human Histone H4

What are the excitation and emission wavelengths of the AbBy Fluor-750 conjugate (bs-23810R-BF750)?

AbBy Fluor-750 on bs-23810R-BF750: excitation / emission 749nm/775nm.