Semaphorin 3F, Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-23827R-Cy5.5 |
| Product Name | Semaphorin 3F, Cy5.5 Conjugated |
| Applications |
WB
IF
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Dog, Cow, Sheep, Pig, Chicken, Rabbit |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Semaphorin 3F |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1mg/ml |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 6405 |
| Swiss Prot | Q13275 |
| Subcellular location | Secreted |
| Synonyms | Semaphorin-3F; Semaphorin3F; Sema III/F; Sema IV; sema3F; SemaIII/F; SemaIV; Semaphorin IV; SemaphorinIV; SEM3F_HUMAN. |
| Background | May play a role in cell motility and cell adhesion. |
| Application Dilution | |
| WB | =1:500-2000 |
| IF | |
Frequently asked questions
Which applications is Semaphorin 3F, Cy5.5 Conjugated (BS-23827R-CY5-5) validated for, and at what dilution?
BS-23827R-CY5-5 is validated for WB (WB=1:500-2000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Semaphorin 3F, Cy5.5 Conjugated (BS-23827R-CY5-5) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Cow, Sheep, Pig, Chicken, Rabbit.
How should BS-23827R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Semaphorin 3F, Cy5.5 Conjugated (BS-23827R-CY5-5)?
KLH conjugated synthetic peptide derived from human Semaphorin 3F
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-23827R-CY5-5)?
Cy5.5 on BS-23827R-CY5-5: excitation / emission 675nm/694nm.