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Semaphorin 3F, FITC Conjugated

Applications

  • WB
  • IF

Reactivity

  • Mouse
  • Rat

Predicted Reactivity

  • Human
  • Dog
  • Cow
  • Sheep
  • Pig
  • Chicken
  • Rabbit
Overview
Catalog # bs-23827R-FITC
Product Name Semaphorin 3F, FITC Conjugated
Applications
WB IF
Reactivity Mouse, Rat
Predicted Reactivity Human, Dog, Cow, Sheep, Pig, Chicken, Rabbit
Specifications
Conjugation FITC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Semaphorin 3F
Clonality Polyclonal
Isotype IgG
Concentration 1mg/ml
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 6405
Swiss Prot Q13275
Subcellular location Secreted
Synonyms Semaphorin-3F; Semaphorin3F; Sema III/F; Sema IV; sema3F; SemaIII/F; SemaIV; Semaphorin IV; SemaphorinIV; SEM3F_HUMAN.
Background May play a role in cell motility and cell adhesion.
Application Dilution
WB =1:500-2000
IF

Frequently asked questions

Which applications is Semaphorin 3F, FITC Conjugated (bs-23827R-FITC) validated for, and at what dilution?

bs-23827R-FITC is validated for WB (WB=1:500-2000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Semaphorin 3F, FITC Conjugated (bs-23827R-FITC) react with?

Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Cow, Sheep, Pig, Chicken, Rabbit.

How should bs-23827R-FITC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Semaphorin 3F, FITC Conjugated (bs-23827R-FITC)?

KLH conjugated synthetic peptide derived from human Semaphorin 3F

What are the excitation and emission wavelengths of the FITC conjugate (bs-23827R-FITC)?

FITC on bs-23827R-FITC: excitation / emission 494nm/518nm.