Coxsackie Adenovirus Receptor Antibody, APC-Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-2389r-apc-cy7-100ul |
| Product Name | Coxsackie Adenovirus Receptor Antibody, APC-Cy7 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Dog, Cow, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | APC-Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Coxsackie Adenovirus Receptor |
| Immunogen Range | 21-120/365 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1525 |
| Swiss Prot | P78310 |
| Subcellular location | Secreted, Cell membrane |
| Synonyms | CAR; HCAR; CAR4/6; Coxsackievirus and adenovirus receptor; CVB3-binding protein; Coxsackievirus B-adenovirus receptor; HCVADR; CXADR |
| Background | Component of the epithelial apical junction complex that may function as an homophilic cell adhesion molecule and is essential for tight junction integrity. Also involved in transepithelial migration of leukocytes through adhesive interactions with AMICA1/JAML a transmembrane protein of the plasma membrane of leukocytes. The interaction between both receptors also mediates the activation of gamma-delta T-cells, a subpopulation of T-cells residing in epithelia and involved in tissue homeostasis and repair. Upon epithelial CXADR-binding, AMICA1 induces downstream cell signaling events in gamma-delta T-cells through PI3-kinase and MAP kinases. It results in proliferation and production of cytokines and growth factors by T-cells that in turn stimulate epithelial tissues repair. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Coxsackie Adenovirus Receptor Antibody, APC-Cy7 Conjugated (bs-2389R-APC-Cy7) validated for, and at what dilution?
bs-2389R-APC-Cy7 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Coxsackie Adenovirus Receptor Antibody, APC-Cy7 Conjugated (bs-2389R-APC-Cy7) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Cow, Pig, Horse, Rabbit.
How should bs-2389R-APC-Cy7 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Coxsackie Adenovirus Receptor Antibody, APC-Cy7 Conjugated (bs-2389R-APC-Cy7)?
KLH conjugated synthetic peptide derived from human Coxsackie Adenovirus Receptor Immunogen range: 21-120/365.
What are the excitation and emission wavelengths of the APC-Cy7 conjugate (bs-2389R-APC-Cy7)?
APC-Cy7 on bs-2389R-APC-Cy7: excitation / emission 650nm/780nm.