Smad4 Polyclonal Antibody

$Lane 1: Human K562 cell lysates; Lane 2: Human Hela cell lysates; Lane 3: Human Jurkat cell lysates; Lane 4: Human HL-60 Cell lysates probed with Smad4 Polyclonal Antibody, Unconjugated (bs-23966R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Human K562 cell lysates; Lane 2: Human Hela cell lysates; Lane 3: Human Jurkat cell lysates; Lane 4: Human HL-60 Cell lysates probed with Smad4 Polyclonal Antibody, Unconjugated (bs-23966R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA

Reactivity

Human

Predicted Reactivity

Mouse
Rat
Dog
Cow
Sheep
Pig
Horse
Chicken
Rabbit
Zebrafish

Overview
Catalog #	bs-23966R
Product Name	Smad4 Polyclonal Antibody
Applications	WB, ELISA
Reactivity	Human
Predicted Reactivity	Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit, Zebrafish
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Smad4
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1mg/ml
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	4089
Swiss Prot	Q13485
Subcellular location	Cytoplasm, Nucleus
Synonyms	Mothers against decapentaplegic homolog 4; (Small) Mothers Against Decapentaplegic; Deleted in Pancreatic Carcinoma; Deleted in pancreatic carcinoma locus 4; Deletion target in pancreatic carcinoma 4; DPC 4; DPC4; hSMAD4; JIP; MAD homolog 4; MAD mothers against decapentaplegic Drosophila homolog 4; MAD mothers against decapentaplegic homolog 4; MADH 4; MADH4; Med; Medea; Mothers against decapentaplegic homolog 4; Mothers against DPP homolog 4; OTTHUMP00000163548; Smad 4; SMAD family member 4; SMAD mothers against DPP homolog 4.
Background	Common SMAD (co-SMAD) is the coactivator and mediator of signal transduction by TGF-beta (transforming growth factor). Component of the heterotrimeric SMAD2/SMAD3-SMAD4 complex that forms in the nucleus and is required for the TGF-mediated signaling. Promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. Component of the multimeric SMAD3/SMAD4/JUN/FOS complex which forms at the AP1 promoter site; required for syngernistic transcriptional activity in response to TGF-beta. May act as a tumor suppressor. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000